| 小鼠脊髓挫伤型损伤后caspase-3、bax、bcl-2和c-kit基因的表达 |
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| 引用本文： | 范睿,张昱,许家玉,鲁亚平,蔡亚非.小鼠脊髓挫伤型损伤后caspase-3、bax、bcl-2和c-kit基因的表达[J].南京农业大学学报,2010,33(1). |
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| 作者姓名： | 范睿 张昱 许家玉 鲁亚平 蔡亚非 |
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| 作者单位： | 1. 安徽师范大学生命科学学院/生物环境与生态安全安徽省高校省级重点实验室,安徽芜湖,241000
2. 安徽省无为县动物疫病预防与控制中心,安徽无为,238300 |
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| 基金项目： | 科技部“十一五”国家科技支撑计划项目(2006BAD04A12);;安徽省高校省级自然科学研究重点项目(KJ2009A039);;芜湖市科技计划重点项目(2008620) |
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| 摘 要： | 为揭示凋亡相关基因和c-kit基因在脊髓损伤(spinal cord injury,SCI)后的相互关系,利用RT-PCR和免疫组化技术,分别检测了caspase-3、bax、bcl-2、c-kit的mRNA和蛋白在小鼠脊髓损伤后的表达。结果显示:在SCI后4 h开始出现大量的神经元和胶质细胞凋亡,其中促凋亡因子表达增加,而抑制凋亡因子表达量减少。caspase-3的mRNA和蛋白的表达在SCI后开始增加,并在72 h时达到峰值,bax表达规律与之基本一致,但是其峰值出现在24 h。随着时间的变化,bcl-2与c-kit的表达均表现为升高—降低—升高的变化趋势,与caspase-3和bax的变化趋势相反,且其调控作用均发生在caspase-3之前。此外,SCI后c-kit基因也呈现抑制凋亡的作用。表明:损伤是导致脊髓发生凋亡的因素之一;bcl-2是凋亡抑制因子,与bax共同控制细胞凋亡,并直接作用于下游caspase-3的表达。
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| 关 键 词： | 凋亡相关基因 c-kit基因 脊髓损伤 表达 |
The expression of caspase-3,bax,bcl-2 and c-kit after spinal cord injury in mouse |
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| FAN Rui,ZHANG Yu,XU Jia-yu,LU Ya-ping,CAI Ya-fei.The expression of caspase-3,bax,bcl-2 and c-kit after spinal cord injury in mouse[J].Journal of Nanjing Agricultural University,2010,33(1). |
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| Authors: | FAN Rui ZHANG Yu XU Jia-yu LU Ya-ping CAI Ya-fei |
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| Institution: | 1*;1.College of Life Sciences/Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province/a>;Anhui Normal University/a>;Wuhu 241000/a>;China/a>;2.Anhui Wuwei Animal Disease Prevention and Control Centre/a>;Wuwei 238300/a>;China |
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| Abstract: | To characterize the reciprocity mechanism of four genes in spinal cord injury(SCI),semi-quantitative RT-PCR and immunohistochemistry technology were implied to investigate the expression of caspase-3,bax,bcl-2 and c-kit after SCI in KM mouse. The results were as follows:A great of apoptosis cells were observed at 4 h after SCI,in which the expression of promoting genes increased and restraining genes reduced.The expression of caspase-3 mRNA and protein increased to peak at 72 h after SCI,and this pattern wa... |
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| Keywords: | genes due to apoptosis c-kit gene spinal cord injury expression |
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