眼斑拟石首鱼暴发性传染病病原初步研究

1999年 8～ 10月广东沿海网箱养殖的眼斑拟石首鱼 (Sciaenopocellatus)流行一种暴发性传染病 ,经抗菌药或杀虫剂处理均无效。为查明病因 ,取病鱼镜检 ,未发现寄生虫 ,也分离不到细菌。电镜观察 ,可见病鱼的脾脏细胞内有截面为六边形、直径约 15 0nm的病毒颗粒 ,且脾、肝、肾都有一定程度的病变 ;用 2对日本所报道的虹彩病毒特异引物进行PCR扩增 ,在病鱼的脾脏样品中扩增出 2条大小与所报道的虹彩病毒扩增产物吻合的特征带 ,故初步认定虹彩病毒是眼斑拟石首鱼暴发性传染病的主要致病原

Pathogen of infective outbreaking disease in Sciaenop ocellatus

From Aug. to Oct.1999, a kind of infective outbreaking disease occured in seawater cage cultured Sciaenop ocellatus along Guangdong coastal sea. Neither parasites were found on body surface, gill filaments and other organs by microscope examination, nor pathogenis bacteria isolated from internal organs (such as liver and kidney). However, under the electron microscope, a lot of virus particles were observed, and the virion was hexagon in section and about 150 nm in diameter. Some pathological changes were found in internal organs (speen, liver, kidney). Using two pairs of PCR primers of Iridovirus reported by Jun Kurita, a single DNA band was amplified with each pair of primers, and the expected molecular size was similar to that reported. It indicates that the major pathogen of the infective outbreaking disease in the Sciaenop ocellatus is Iridovirus.