Seroprevalence of antibodies to Salmonella spp in semidomesticated reindeer in Norway,determined by enzyme-linked immunosorbent assay

An indirect ELISA was developed as a possible tool to detect the seroprevalence of antibodies to Salmonella spp in semidomesticated reindeer. To cover a broad spectrum of serogroups a lipopolysaccharide mix of S. typhimurium and S. choleraesuis was used as antigen in this pilot study. Sera from 31 culture-negative reindeer with no clinical or historical evidence of salmonellosis were used as negative serum control. After immunisation with an inactivated S. typhimurium vaccine, pooled sera from 6 reindeer were used as positive serum control as no serum from naturally infected animals was available. A seroprevalence of 0.6% in 2000 clinically healthy, slaughter-reindeer from Norway was determined by using this ELISA. No more information on Salmonella in reindeer in Norway is known to the authors. This is the first ELISA established for indirect detection of Salmonella in reindeer.