| 蕨菜愈伤组织高效再生体系的建立 |
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| 引用本文： | 姜长阳,宁淑香,于淼,王宇,宋立秀. 蕨菜愈伤组织高效再生体系的建立[J]. 园艺学报, 2003, 30(3): 343-345 |
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| 作者姓名： | 姜长阳 宁淑香 于淼 王宇 宋立秀 |
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| 作者单位： | (辽宁师范大学生命科学学院, 大连 116029) |
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| 摘 要： | 报告了蕨菜不同外植体在含有不同激素的培养基上诱导、分化及生根情况，建立起高效再生体系。诱导愈伤组织的最适培养基为1/2 MS+BA 0.2 mg/L+IBA 0.4 mg/L+NH4H2PO4 200 mg/L; 诱导愈伤组织芽分化的最适培养基为1/2 MS，芽分化率为100％; 不定苗在1/2 MS+IAA 0.2 mg/L 培养基上生根率为100％，同时可以形成健壮根茎；具有根茎的试管苗移栽成活率达94％。
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| 关 键 词： | 蕨菜 组织培养 植株再生 |
| 文章编号： | 0513-353X(2003)03-0343-03 |
| 收稿时间： | 2002-10-31 |
| 修稿时间： | 2003-02-11 |
A High Efficient Plant Regeneration System from Callus of Pteridium apuilinum |
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| Jiang Changyang,Ning Shuxiang,Yu Miao,Wang Yu,and Song Lixiu. A High Efficient Plant Regeneration System from Callus of Pteridium apuilinum[J]. Acta Horticulturae Sinica, 2003, 30(3): 343-345 |
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| Authors: | Jiang Changyang Ning Shuxiang Yu Miao Wang Yu and Song Lixiu |
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| Affiliation: | ( College of Life Science, Liaoning Normal University, Dalian 116029，China) |
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| Abstract: | Induction,differentiation and rooting of different explants of Pteridium apuilinum were described on different medium in which contained different hormones and a high efficient plant regeneration system was established. The result showed that the most suitable medium was 1/2 MS+0.2mg/L BA+0.4mg/L IBA+200mg/L NH 4H 2PO 4;the induced frequency of bud from callus was 100% on medium in contained 1/2 MS;The radiation rate of adventitious seedling was 100% on medium 1/2 MS+0.2mg/L IAA,simultaneously vigorous rhizome was formed and the survival rate of transplantation of test-tube seedling could reach a high level at 94%. |
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| Keywords: | Pteridium aquilinum(L.)Kuhn. Tissue culture Plant regeneration |
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