| Abstract: | AIM: To investigate the expression and location of SET domain-containing 4(SETD4) protein in p38 +/+ and p38 -/- cells treated with sodium arsenite(NaAsO2). METHODS: The expression and location of SETD4 were detected in different cells with or without NaAsO2 treatment by Western blotting and immunofluorescence technique. RESULTS: The expression of SETD4 was detectable in both murine- and human-derived cells. Its distribution was found to be located in the whole cell, mainly in the cytoplasm. Further investigation also suggested that the protein expression of SETD4 was reduced in both p38 +/+ and p38 -/- cells 6 h after NaAsO2 treatment. Moreover, SETD4 protein was translocated from cytoplasm to nucleus in p38 +/+ cells treated with NaAsO2, which was unobvious in p38 -/- cells. CONCLUSION: SETD4 protein is expressed in various cells derived from different species and tissues, and it is mainly located in cytoplasm. NaAsO2 treatment influences the expression of SETD4, and induces the translocation of SETD4 protein to nucleus, which might be involved in the p38 MAPK signal pathway. |
