Balance of Treg/Th17 in synovium of collagen-induced arthritis rats and impact of TNF-α blockage therapy |
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Authors: | CHEN Rui-lin TAO Yi HUANG Wen-hui L Zhi-fen LI Juan |
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Affiliation: | 1. Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;2. Department of Rheumatology & Immunology, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, China |
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Abstract: | AIM: To investigate the balance of Treg/Th17 in synovium of collagen-induced arthritis (CIA) and the impact of tumor necrosis factor α(TNF-α) blockage therapy. METHODS: Rat CIA model was established by bovine II collagen injection. The pathological score was evaluated by HE staining and toluidine blue staining. The TNF-α level in plasma was measured by ELISA. The expression of Treg/Th17 in synovium was detected by double staining immunofluorescence. RESULTS: The plasma level of TNF-α in CIA group was significantly higher than that in control group and TNFR-Fc treatment group (P<0.01), whereas no significant difference was found between TNFR-Fc treatment group and control group (P>0.05). No significant difference between CIA group and control group in the ratio of CD4+Foxp3+Treg cells/CD4+ cells in synovium (23.12%±4.93% vs 24.66%±5.82%, P>0.05) was observed, whereas the ratio in TNFR-Fc treatment group was significantly increased(33.07%±5.14%). The ratio of CD4+RORγt+Th17 cells/CD4+ cells in CIA group was significantly higher than that in control group and TNFR-Fc treatment group (9.74%±2.23% vs 1.00%±0.59%, 5.63%±1.76%, P<0.01). CONCLUSION: Differentiation disturbance of Treg/Th17 exists in the synovium of CIA rats. TNFR-Fc may restore the balance of Treg/Th17 by inhibiting Th17 cell differentiation and inducing the production or accumulation of Treg. |
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Keywords: | Collagen-induced arthritis T-lymphocytes regulatory Th17 cells Tumor necrosis factor &alpha antagonist |
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