Association of endothelial nitric oxide synthase gene rs7830 and rs3918188 polymorphisms with essential hypertension in Kazakh of Xinjiang region

AIM: To investigate the correlation between endothelial nitric oxide synthase (eNOS) rs7830 and rs3918188 locus polymorphisms and essential hypertension (EH) in the Kazakh of Xinjiang region. METHODS: Epidemiological case-control study was conducted. DNA was extracted by classic phenol-chloroform method, PCR amplification and purification. The rs7830 and rs3918188 of eNOS gene in 363 EH patients (EH group) and 370 normotensive controls (NT group) in the Kazakh of Xinjiang region were genotyped by the technique of SNaPshot single nucleotide polymorphism genotyping. The plasma levels of fasting blood glucose, uric acid, cholesterol and triglyceride were measured by biochemical methods. Determination of body mass index and waist-hip ratio was also conducted. RESULTS: Age (P<0.01), body mass index (P<0.01), triglyceride (P<0.01), low-density lipoprotein (P<0.05) and apolipoprotein A1/B (P<0.05) were the independent factors for EH in the Kazakh of Xinjiang region. No difference of eNOS gene rs7830 and rs3918188 loci in the genotype frequency and the allele frequency distribution between EH patients and normotensive controls in the Kazakh of Xinjiang region was observed (P>0.05). The frequency distribution of CA, CC, AC and AA haplotypes from eNOS gene rs7830 and rs3918188 loci between EH group and NT group also had no difference in the Kazakh of Xinjiang region (P>0.05). CONCLUSION: Age, body mass index and triglyceride are the independent risk factors, while low-density lipoprotein and apolipoprotein A1/B are the independent protective factors for EH in the Kazakh of Xinjiang region. The polymorphisms of eNOS gene rs3918188 and rs7830 loci are not related to EH in the Kazakh of Xinjiang region.