Lipoxin A4 reduces lipopolysaccharide-induced expression of cyclooxygenase 2 and prostaglandin E2 in human bronchial epithelial cells |
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Authors: | ZHU Tian-qi ZHENG Sheng-xing JIN Sheng-wei |
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Institution: | 1. Department of Anesthesia, The First Affiliated Hospital of Wenzhou Medical College, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China;2. Department of Anesthesia, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China;3. Intensive Care Unit, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China |
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Abstract: | AIM: To explore the effects of lipoxin A4 on the expression of cyclooxygenase 2 (COX-2) in human bronchial epithelial cells (HBECs). METHODS: HBECs were incubated with various concentrations (0.1, 1 and 10 mg/L) of lipopolysaccharide(LPS) for 9 h, or 1 mg/L LPS for different time (3 h, 6 h and 9 h). The levels of COX-2 mRNA in HBECs and prostaglandin E2 (PGE2) in the culture supernatant were measured. In addition, the HBECs were exposed to lipoxin A4 at concentration of 0, 100 and 400 μmol/L after stimulated with LPS at concentration of 1 mg/L for 9 h, and the supernatant of the culture cells was collected for determining the content of PGE2 by ELISA. The cells were also harvested, and the mRNA and protein levels of COX-2 were analyzed by RT-PCR and Western blotting, respectively. RESULTS: LPS increased the mRNA expression of COX-2 and production of PGE2 in a dose and time dependent manners in HBECs. Induction of COX-2 mRNA and protein by LPS were inhibited by lipoxin A4 in a dose-dependent manner. Lipoxin A4 also significantly decreased LPS-induced production of PGE2. CONCLUSION: Lipoxin A4 down-regulates LPS-induced expression of COX-2 and consequently inhibits the production of PGE2 in HBECs. |
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Keywords: | Lipoxin A4 Human bronchial epithelial cells Cyclooxygenase 2 Lipopolysaccharides |
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