Effect of microtubule-destabilizing protein stathmin gene silencing on proliferation and apoptosis of nasopharyngeal carcinoma cell line 5-8F |
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Authors: | LEI Xun FAN Cai-wen TIAN Jing XIAO Sheng-jun WANG Jian-hong LIU Qiang-he XIANG Qiu |
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Institution: | 1. Department of Otorhinolaryngology, the Affiliated Hospital, Guilin Medical College, Guilin 541001, China;2. Department of Pathology and Pathophysiology, Guilin Medical College, Guilin 541001, China |
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Abstract: | AIM: To investigate the effects of stathmin gene silencing on nasopharyngeal carcinoma cell line 5-8F. METHODS: Double-strand siRNA targeting to stathmin gene was obtained by chemical synthesis and annealing, and was sub-cloned into the vector pGenesil-1.1. The plasmid was introduced into 5-8F cells by liposome-mediated transfection. The gene expression of stathmin, and the proliferation, morphology and apoptosis of the cells were analyzed by Western blotting, MTT assay and flow cytometry. RESULTS: The cell suppression rate in stathmin gene silencing group was (53.01?1.12)%, significantly higher than that in transfection reagent group and in negative control group. The cell apoptotic rate in stathmin gene silencing group was (8.75?0.67)%, also significantly higher than that in transfection reagent group and in negative control group (P<0.05). CONCLUSION: Silencing of stathmin gene in nasopharyngeal carcinoma cells inhibits the cell proliferation and induces cell apoptosis. |
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Keywords: | Stathmin RNA interference Nasopharyngeal neoplasms |
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