硫胁迫对油菜超微结构及超细胞水平硫分布的影响

利用温室砂培盆栽试验，结合油菜缺硫症状的形态表现特征，进行了硫胁迫条件下油菜营养与生殖器官超微结构及超细胞水平硫素分布的电子探针分析。结果表明，硫胁迫导致油菜叶片皱缩，叶脉聚集、扭曲、隆起，气孔张开、变形，叶肉细胞叶绿体基粒片层松弛、肿胀，淀粉积累；花瓣细胞质发育不良，细胞内含物颗粒呈聚集状；柱头细胞器排列不规则，质膜呈断续状，细胞壁松弛、增厚；花粉粒发育不全，形状不规则，多呈扁瘪、畸形，纹饰不整齐，条脊不规则，穿孔大小不均，花粉壁发育不完善，淀粉粒出现积累并呈复合状。缺硫还显著降低油菜超细胞水平的硫素分布，尤其对叶绿体、生物膜及线粒体等的影响更严重。

EFFECT OF SULFUR STRESS ON ULTRASTRUCTURE AND SULFUR DISTRIBUTION IN VEGETATIVE AND REPRODUCTIVE PARTS OF OILSEED RAPE

The ultrastructures and sulfur distribution of vegetative and reproductive parts and flowers with sulfur stress of oilseed rape grown in sand culture were studied by using SEM and TEM connected with X ray microanalyzer (XMA) The results indicated that sulfur stress resulted in leaf blade shrink, controlling function of stoma on and off lost, grana lamella of chloroplast loose and expansion (turgor), external menbrane destroied, starch accumulated, petal plasma hypoplasia, ropiness decreased; organella in stigmatic cell of S deficient were in desorder, plasma membrane wall in complete, cell wall became loose and thick; pollen grains were squat shaped, shrunken and deformity, pores were uneven, texture unregular and starch accumulated in the pollen cells with sulfur stress Therefore, it fully indicated the importance of sulfur nutrition for maintaining the cell normal structures and metabolic functions of plants