Methodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americana M.) |
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| Authors: | Uri Lavi Mahinur Akkaya Arvind Bhagwat Emanuel Lahav Perry B Cregan |
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| Institution: | (1) Institute of Horticulture, ARO, Volcani Center, P.O. Box 6, 50250 Bet-Dagan, Israel;(2) Soybean and Alfalfa Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Center, 20705-2350 Beltsville, Maryland, USA;(3) Present address: Biological Carcinogenesis and Development Program, Program Resources Inc., Frederick Research Facility, 21701 Frederick, Maryland, USA |
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| Abstract: | Summary Generation of Simple Sequence Repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequences flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR A1E11 was found to have eleven alleles while A3F8 has eight alleles. The SSRs in avocado were found to be inherited in a Mendelian fashion.Contribution from the Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel. No. 1335-E, 1994 series. |
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| Keywords: | breeding genetics microsatellites PCR SSR avocado Persea americana |
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