LPS诱导仔猪腓肠肌相关microRNA在不同时间点的mRNA表达

为研究脂多糖(LPS)刺激后不同时间点断奶仔猪腓肠肌相关microRNA(miRNA)基因的变化规律,试验选取(7.1±0.9)kg杜×长×大断奶仔猪42头,随机分为7个处理组,每个处理6头猪,分别于注射LPS之前(0 h)和注射LPS之后1、2、4、8、12、24 h,屠宰仔猪,取腓肠肌测定相关miRNA的表达。结果表明:与对照组相比,LPS刺激后2 h,miRNA-10b的mRNA表达量显著降低(P<0.01),且于12 h达到峰值;LPS刺激后1 h,miRNA-1和miRNA-206的mRNA表达量均显著降低(P<0.01),但miRNA-133a的mRNA表达量无显著变化(P>0.05)。LPS刺激早期可诱导腓肠肌相关基因miRNA-10b、miRNA-1、miRNA-206的m RNA表达量在不同时间点下调,表明这些miRNA可能参与了肌肉炎症早期相关信号通路的调控。

LPS induced mRNA expression of gastrocnemius related microRNA in piglets at different time points

The purpose of this study was to investigate the changes of microRNA(miRNA)gene in gastrocnemius muscle of weaned piglets at different time points after lipopolysaccharide(LPS)stimulation.Forty two(7.1±0.9)kg Duroc×Landrace×Yorkshine weaned piglets were randomly divided into seven treatment groups,six pigs in each treatment group.The piglets were slaughtered before(0 h)and 1,2,4,8,12,24 h after LPS injection,and the expression of miRNA was measured in gastrocnemius muscle.The results showed as follows:Compared with the control group,the mRNA expression of miRNA-10 b decreased significantly at 2 h after LPS stimulation(P<0.01),and reached the peak at 12 h;1 h after LPS stimulation,the mRNA expression of miRNA-1 and miRNA-206 decreased significantly(P<0.01),but the mRNA expression of miRNA-133 a did not change significantly after LPS stimulation(P>0.05).It indicated that LPS could induce the mRNA expression of miRNA-10 b,miRNA-1 and miRNA-206 in gastrocnemius muscle at different time points,these results suggested that these miRNAs may be involved in the regulation of early inflammatory signaling pathways.