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| Roseomonas JS018有机磷农药降解酶的纯化 | |
| 引用本文： | 江玉姬,邓优锦,刘新锐,胡方平,谢宝贵,刘福阳,黎志银.Roseomonas JS018有机磷农药降解酶的纯化[J].福建农林大学学报(自然科学版),2006,35(4):421-425. |
| 作者姓名： | 江玉姬 邓优锦 刘新锐 胡方平 谢宝贵 刘福阳 黎志银 |
| 作者单位： | 1. 福建农林大学食品科学学院;福建农林大学植物保护学院 2. 福建农林大学生命科学学院,福建,福州,350002 3. 福建农林大学植物保护学院 |
| 基金项目： | 引进国际先进农业科技计划(948计划) |
| 摘 要： | 本试验对能高效降解几种有机磷农药菌株JS018的降解酶进行分离和纯化.经分析该降解酶主要位于细胞间质,最适盐析硫酸铵饱和度为60%-70%,粗酶液经硫酸铵沉淀、Sephadex G-100凝胶过滤和DEAE-52柱层析得到较高纯度的酶液.通过SDS-PAGE电泳测定该酶相对分子质量约为37 ku,N末端16个氨基酸残基测序结果为:GAPFQNDVPPACYRFR. |
| 关 键 词： | 有机磷农药降解酶 电泳 层析 纯化 测序 |
| 文章编号： | 1671-5470(2006)04-0421-05 |
| 修稿时间： | 2005年12月19 |
Purification of the organophosphorus pesticide degrading enzyme from Roseomonas JS018 |
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| JIANG Yu-ji,DENG You-jin,LIU Xin-rui,HU Fang-ping,XIE Bao-gui,LIU Fu-yang,LI Zhi-yin.Purification of the organophosphorus pesticide degrading enzyme from Roseomonas JS018[J].Journal of Fujian Agricultural and Forestry University,2006,35(4):421-425. | |
| Authors: | JIANG Yu-ji DENG You-jin LIU Xin-rui HU Fang-ping XIE Bao-gui LIU Fu-yang LI Zhi-yin |
| Abstract: | The organophosphorus pesticide degrading enzyme produced from strain JS018 was purified by ammonium sulfate precipitation,Sephadex G-100 gel filtration and chromatography on DEAE-52.It mainly located in periplasmic space.The test results showed that the optimal concentration of ammonium sulfate was 60%-70%.The molecular weight of the enzyme was about 37 ku by SDS-PAGE.The N-terminal amino acid sequence was determined as follows: GAPFQNDVPPACYRFR. |
| Keywords: | organophosphorus pesticide degrading enzyme electrophoresis chromatography purification sequence |
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