| 基因克隆技术 |
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| 引用本文： | 周国岭,杨光圣,等.基因克隆技术[J].华中农业大学学报,2001,20(6):584-592. |
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| 作者姓名： | 周国岭 杨光圣 |
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| 作者单位： | 华中农业大学 |
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| 摘 要： | 综述了基因克隆常用技术包括图位克隆法、转座子或T－DNA标签法、同源序列法、表达序列标签（EST）法和差异表达基因分离方法的原理、应用、应用的潜力和限制因素。
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| 关 键 词： | 基因克隆 图位克隆 转座子标签 T－DNA标签 同源序列 EST 差异表达基因 |
| 修稿时间： | 2001年5月4日 |
Gene Cloning Techniques |
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| Zhou Guoling,Yang Guangsheng,Fu Tingdong.Gene Cloning Techniques[J].Journal of Huazhong Agricultural University,2001,20(6):584-592. |
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| Authors: | Zhou Guoling Yang Guangsheng Fu Tingdong |
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| Abstract: | The rationale, application and application potential and some factors of limitation of some important gene cloning techniques were studied in this paper. The gene cloning technigues include map based cloning or positional cloning,transposon or T DNA tagging method,homology based candidate gene method,expressed sequence tagging method and some differentially expressed gene cloning method in this study. |
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| Keywords: | gene cloning map based cloning transposon tagging T DNA tagging homologous sequence expressed sequence tagging differentially expressed gene |
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