Whole-Cell Fatty Acid Composition to Characterize and Differentiate Isolates of Rhizoctonia Species Associated with Turfgrass Diseases in Japan |
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Authors: | Achmadi PRIYATMOJO Ryo YAMAUCHI Koji KAGEYAMA Mitsuro HYAKUMACHI |
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Institution: | (1) United Graduate School of Agricultural Science, Gifu University, Gifu 501–1193, Japan, JP;(2) Faculty of Agriculture, Gifu University, Gifu 501–1193, Japan, JP |
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Abstract: | Cellular fatty acids were analyzed to characterize and differentiate 34 isolates of Rhizoctonia species representing binucleate Rhizoctonia AG-D (I), AG-D (II), R. solani AG 2-2 IIIB, AG 2-2 LP, R. circinata var. circinata and var. oryzae associated with turfgrass diseases in Japan. Myristic, pentadecanoic, palmitic, palmitoleic, stearic, oleic, linoleic and
linolenic acids were consistently present in varying quantities in all isolates. Heptadecanoic and 9-heptadecenoic acids were
present in isolates of Rhizoctonia AG-D (I), AG-D (II), R. solani AG 2-2 IIIB and AG 2-2 LP but not in isolates of R. circinata var. circinata and var. oryzae. Palmitic, oleic and linoleic acids were the major fatty acids found, constituting 88.30-98.37% of the whole-cell fatty acid
content. The remaining fatty acids were present in smaller amounts. Isolates within a single group were closely clustered,
whereas isolates from different groups were clearly distinguishable based on average linkage cluster analysis of cellular
fatty acids. Principal component analysis, based on all fatty acids detected, confirmed the distinct separation of isolates
representing the six groups of Rhizoctonia species obtained from turfgrasses. These results suggested that fatty acid analysis is useful for the characterization and
differentiation of isolates of Rhizoctonia species associated with turfgrass diseases.
Received 21 May 2001/ Accepted in revised form 28 September 2001 |
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Keywords: | : fatty acid binucleate Rhizoctonia AG-D Rhizoctonia circinata Rhizoctonia solani turfgrass |
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