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| 蝴蝶兰组织培养中防褐化技术研究 | |
| 引用本文： | 王立娅,方正,李英丽,王利娜,宋朝辉.蝴蝶兰组织培养中防褐化技术研究[J].河北农业大学学报,2008,31(5). |
| 作者姓名： | 王立娅 方正 李英丽 王利娜 宋朝辉 |
| 作者单位： | 1. 河北农业大学河北省生物无机化学重点实验室,河北,保定,071001 2. 河北省威县农业局,河北,威县,054700 3. 河北保定市园林绿化管理局,河北,保定,071000 |
| 基金项目： | 河北省博士学科点专项科研基金 |
| 摘 要： | 以蝴蝶兰‘Dps.Jetgreen Mantefon’品种为试材,以继代培养获得的丛生芽为外植体,系统研究了2种吸附剂—活性炭(AC)、聚乙烯吡咯烷酮(PVP)及不同预处理(外植体4℃冷藏24 h、黑暗预处理5 d和黑暗预处理10 d)对蝴蝶兰组培中组织褐化的影响。结果表明:活性炭能有效防止褐化和促进生长,综合效果最佳。除外植体4℃冷藏24 h处理外,其余处理措施均可减轻组织褐化,但效果均不及活性炭。 |
| 关 键 词： | 蝴蝶兰 外植体 褐化 预处理 |
Study on browning of Phalaenopsis amabilis in the tissue culture |
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| WANG Li-ya,FANG Zheng,LI Ying-li,WANG Li-na,SONG Zhao-hui.Study on browning of Phalaenopsis amabilis in the tissue culture[J].Journal of Agricultural University of Hebei,2008,31(5). | |
| Authors: | WANG Li-ya FANG Zheng LI Ying-li WANG Li-na SONG Zhao-hui |
| Abstract: | The clustered shoots generated from proliferation period of Phalaenopsis amabilis were used as explant in order to study the browning of tissue culture.The treatments include two adsorbents: active carbon,PVP,and three pretreatments: explants preserved at 4℃ for 24 hours,with darkness pretreatment for 5 days and 10 days.The results indicated that active carbon was the best one of all the treatments,which could control browning and promote explant growth.Except for the explants preserved at 4℃ for 24 hours,the other treatments could decrease browning,but the effects were not as good as active carbon. |
| Keywords: | Phalaenopsis amabilis explant browning pretreatment |
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