| 鹿茸间充质细胞全长cDNA文库的构建 |
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| 引用本文： | 施安国,赵海平,邢秀梅,杨颖,鲁晓平,杨福合. 鹿茸间充质细胞全长cDNA文库的构建[J]. 特产研究, 2010, 32(4): 9-12 |
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| 作者姓名： | 施安国 赵海平 邢秀梅 杨颖 鲁晓平 杨福合 |
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| 作者单位： | 中国农业科学院特产研究所国家特种经济动物分子生物学重点试验室吉林省特种经济动物分子生物学重点试验室中国农业科学院特种经济动物遗传改良重点开放试验室; |
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| 基金项目： | 国家自然科技资源平台项目 |
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| 摘 要： | 解剖梅花鹿鹿茸,取得间充质,经过胰酶消化,体外培养鹿茸间充质细胞。利用TRIZOL提取鹿茸间充质细胞总RNA,利用BD SMART技术构建鹿茸间充质细胞全长cDNA文库,测定该文库初级文库滴度为3.0×105 pfu/mL,扩增文库滴度为6.0×108 pfu/mL。插入片段长度大于500 bp。
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| 关 键 词： | 鹿茸 间充质 细胞培养 cDNA文库 |
Construction of a Full-length cDNA Library of Antler Mesenchymal Cell in Sika Deer |
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| SHI An-guo,ZHAO Hai-ping,XING Xiu-mei,YANG Ying,LU Xiao-ping,YANG Fu-he. Construction of a Full-length cDNA Library of Antler Mesenchymal Cell in Sika Deer[J]. Special Wild Economic Animal and Plant Research, 2010, 32(4): 9-12 |
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| Authors: | SHI An-guo ZHAO Hai-ping XING Xiu-mei YANG Ying LU Xiao-ping YANG Fu-he |
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| Affiliation: | SHI An-guo,ZHAO Hai-ping,XING Xiu-mei,YANG Ying,LU Xiao-ping,YANG Fu-he(Institute of Special Wild Economic Animals and Plants,CAAS,State Key Laboratory for Molecular Biology of Special Economical Animals,Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals,Key Laboratory for Germlasm Resources Genetic Improvement of Special Economic Animals,Jilin 132109,China) |
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| Abstract: | This experiment obtained the mesenchyme from the antler by the histology dissection.Then we digested the material with the trypsin and cultured the antler mesenchymal cells.Subsequently,total RNA was isolated form antler mesenchymal cells with TRIZOL,and a full-length cDNA library of antler mesenchymal cells was constructed.The titer of the primary library was up to 3.0×105 pfu / mL,after amplified,the titer was 6.0×108 pfu / mL.Insert fragments＇ length were more than 500 bp. |
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| Keywords: | Antler Mesenchyme Cell culture cDNA library |
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