丁香假单胞菌大豆致病变种ICMP2189中乙烯合成酶基因的克隆及其在大肠杆菌中的表达 |
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引用本文: | 董红军,伍丽娴,陈三凤.丁香假单胞菌大豆致病变种ICMP2189中乙烯合成酶基因的克隆及其在大肠杆菌中的表达[J].农业生物技术学报,2007,15(4). |
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作者姓名: | 董红军 伍丽娴 陈三凤 |
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作者单位: | 中国农业大学生物学院微生物与免疫学系陈三凤教授实验室 |
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摘 要: | 本论文通过PCR的方法从丁香假单胞菌大豆致病变种(Pseudomonas syringae pv. glycinea)ICMP2189中克隆到了乙烯合成酶基因efe,并且在大肠杆菌BL21(DE3)中利用T7强启动子进行了高效表达,表达蛋白占总蛋白的45%。气相色谱分析表明含有efe基因的大肠杆菌可以高效的产生乙烯。研究结果为生物乙烯的研究和应用奠定了基础。
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关 键 词: | 丁香假单胞菌大豆致病变种,乙烯合成酶基因(efe),克隆,表达,生物乙烯 |
收稿时间: | 2006-11-1 |
修稿时间: | 2007-1-15 |
Molecular cloning and expression of ethylene-forming enzyme (EFE) gene from Pseudomonas syringae pv. glycinea ICMP2189 |
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Abstract: | The efe gene encoding ethylene-forming enzyme was PCR amplified from Pseudomonas syringae pv. glycinea ICMP2189. The efe gene was cloned to expression vector pET28a, yielding recombinant plasmid pET28a-efe. Then plasmid pET28a-efe was introduced into Escherichia coli BL21(DE3). SDS-PAGE showed that ethylene-forming enzyme with molecular weight of 45 kDa was effectively expressed in E. coli. Gas chromatography analysis demonstrated that the E.coli strain carrying the efe gene could efficiently produce ethylene. |
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