ISSR在高粱属植物遗传关系研究中的应用

以高粱、杂交高粱、甜高粱、苏丹草、黑高粱和假高粱6种不同高粱属植物为实验材料，利用ISSR分子标记分析其遗传关系。结果表明，这6种高粱属植物在DNA水平上具有较高的遗传多样性。从110条ISSR引物中成功筛选到22条多态性高、稳定性好的引物，共扩增出182条带，其中差异性条带153条，多态条带比率(PPB)为84.0%。基于遗传距离系数的Neighbor-Joining聚类分析可以将6种高粱分为两大组，相互之间的遗传距离为0.25。另外可以根据一些多态性高的引物所扩增得到的品种特异性条带区分所有不同高粱种，如引物IR89、IS16等。这些为研究高粱属植物的分类、鉴定和进化提供了分子生物学方面的理论依据。

Application of ISSR in Genetic Relationship Analysis of Sorghum Species

The genetic relationship among six sorghum species, namely, S. bicolor, S. bicolor×S, sudanese, S. saccharatum, S.sudanese, S. almum, and S. halepense was analyzed by inter-simple sequence repeat (ISSR) method. The results showed that the diversity of sorghum was high at DNA level. Twenty-two primers selected from 110 ISSR primers could amplify 182 clear and reproducible bands, of which 153 bands were polymorphic, accounting for 84.0%. All the sorghum species studied could be distinctly divided into two major groups with the genetic distance level at 0.25 by cluster analysis based on the Neighbor-Joining method. Some primers produced highly polymorphic band patterns in different species, such as IR 89, ISI6, according to them, all the sorghum species used could be identified. The results could be used in classification, identification and evolution of sorghum.