芝麻子叶基因转化受体系统的建立

试验以芝麻栽培种豫芝11号为材料,对子叶丛生芽诱导及植株再生体系以及子叶受体材料对卡那霉素、潮霉素和头孢霉素的天然耐性进行了研究,建立了芝麻子叶基因高频转化受体系统.结果表明,适宜的诱芽培养基为MS+5.0 mg/L 6-BA+1.0 mg/L IAA+5.0 mg/L AgNO3 +1.0 mg/L ABA+3％蔗糖...

Establishment of an Aceeptor System for Gene Transformation in Sesame (Sesamum indicum L.) Cotyledon

To establish the acceptor system for gene transformation in sesame cotyledon, perfect culture medium for bud inducement from sesame cotyledon and plantlet regeneration has been investigated, as well as the endurance points of explants and plantlets on selective agents, i. e., Kanamycin, Hygromycin and Cefotaxime, have been studied deeply. Results showed that suitable differentiation medium was MS containing 5.0 mg/L 6-BA, 1.0 mg/L IAA, 5.0 mg/L AgNO3, 1.0 mg/L ABA, 3% sucrose and 0.8% agar. 6-BA would determine the cotyledon expansion. Moreover, being the key factors for shoot regeneration, ABA and IAA could induce high frequency of adventitious bud formation, and AgNO3 could accelerate remarkable multiple shoots regeneration. During three important stages of cotyledon expansion and bud formation, adventitious bud proliferation, and plantlet root formation in the genetic modification system for Sesamum indicum L., appropriate concentration of Kanamycin are 70 mg/L, 70 mg/L and 20 mg/L, Hygromycin are 40 mg/L, 60 mg/L, 10 mg/L, respectively. In the genetic modification system by A. tumefaciens infection, 500 mg/L Cefotaxime could be chosen, by which A. tumefaciens strain growth could be inhibited when needed, yet sesame explants and shoot regeneration would not impaired.