彩色马铃薯二氢黄酮醇4-还原酶(DFR)基因的克隆及生物信息学分析

花色素苷是影响花色的主要色素,二氢黄酮醇4-还原酶(DFR)基因是花色素苷合成的关键酶基因.本研究以云南地方彩色马铃薯品种‘剑川红’和‘转心乌’,外引品种‘黑美人’为实验材料,通过RT-PCR方法从其块茎表皮中克隆到DFR基因的完整cDNA序列,并进行了生物信息学分析.分析结果显示供试的三个彩色马铃薯的DFR基因编码3...

Cloning and Bioinformatical Analysis of Dihydroflavonol 4-reductase Gene from Pigmented Potato

The main pigment which affects flower colors is the anthocyanin, and dihydroflavonol 4-reductase(DFR) is a key enzyme in anthocyanin synthesis. In this study, the materials were ‘Jianchuanhong’ and ‘Zhuanxinwu’, native to the Yunnan province, and the nonnative black potato variety ‘Heimeiren’. The complete cDNAs of DFR were isolated from their tubers (Solanum tuberosum L.) by RT-PCR and analyzed by tools of bioinformatics. According to sequence analysis, DFR cDNAs encoded an poly peptide composed of 381 amino acid residues and had a complete open reading frame. DFR of three colored potato varieties also had a highly conserved NADP(H)-binding site and substrate specificity site. Homologous alignment indicated that the nucleotide similarity and amino acid homology of three kind of colored potato species is 99.24%, 98.78%, respectively. The amino acid homology of these pigmented potatoes with other solanaceae plants, such as Nicotiana alata and Petunia x hybrida, were more than 86%. Neither DFR of these pigmented potatoes had signal peptide, obvious transmembrane structure region. DFR may be a kind of Hydrophilic protein which probably located in cytoplasm. α-helix and random coil were primary secondary structural components of DFR which belong to NADB-Rossmann superfamily.