Effect of glyphosate on tryptamine production and Sekiguchi lesion formation in rice infected with Magnaporthe grisea |
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Authors: | Atsuko Imaoka Makoto Ueno Junichi Kihara Sakae Arase |
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Affiliation: | (1) The United Graduate School of Agricultural Science, Shimane University, Matsue, Japan;(2) Laboratory of Plant Pathology, Faculty of Life and Environmental Science, Shimane University, Matsue 690-8504, Japan |
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Abstract: | When exposed to light, the Sekiguchi lesion (sl) rice mutant has an enhanced resistance to Magnaporthe grisea infection responsible for Sekiguchi lesion formation and tryptamine accumulation. Glyphosate [N-(phosphonomethyl) glycine] pretreatment suppressed Sekiguchi lesion formation and tryptamine accumulation in the sl mutant after M. grisea infection even under light. This inhibition by glyphosate was blocked by the supply of exogenous tryptophan, but not by exogenous phenylalanine. In glyphosate-pretreated leaves, 5-enol-pyruvyl-shikimate-3-phosphate synthase gene expression and tryptophan biosynthesis were significantly suppressed. During tryptophan starvation, catalase activity was maintained at a high level even under light, leading to the suppression of H2O2 generation and DNA fragmentation. These results show a strong relationship between the tryptophan and tryptamine pathways in the induction of light-enhanced resistance to M. grisea infection in the sl mutant. |
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Keywords: | Glyphosate Tryptophan Sekiguchi lesion Rice Magnaporthe grisea |
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