| HPLC法青风藤根中青藤碱含量的测定及其提取工艺的优化 |
| |
| 引用本文： | 陈曦. HPLC法青风藤根中青藤碱含量的测定及其提取工艺的优化[J]. 安徽农业科学, 2010, 38(29): 16236-16238. DOI: 10.3969/j.issn.0517-6611.2010.29.065 |
| |
| 作者姓名： | 陈曦 |
| |
| 作者单位： | 陕西省资源生物重点实验室,陕西理工学院,陕西汉中,723000 |
| |
| 摘 要： | [目的]测定青风藤根中青藤碱的含量并与藤茎中含量比较,同时优化青藤碱的提取工艺,为合理有效地开发青风藤资源提供依据。[方法]用HPLC法测定青藤碱的含量,通过正交试验优化青藤碱的提取工艺。[结果]青风藤根中青藤碱的平均含量为1.73%,比藤茎中含量高0.62%;青藤碱的最佳提取工艺为：乙醇浓度为70%,乙醇用量为15ml,超声处理时间为30min,超声频率为30Hz。[结论]该试验比较了青风藤根和藤茎中青藤碱的含量差异,为合理有效地开发利用青风藤资源、评价青风藤药材提供了可靠的依据。
|
| 关 键 词： | 青风藤根 青藤碱 含量测定 高效液相法 提取工艺 |
Determination of the Content of Sinomenine in Root of Caulis Sinomenii by HPLC and Purification of Its Extraction Process |
| |
| CHEN Xi. Determination of the Content of Sinomenine in Root of Caulis Sinomenii by HPLC and Purification of Its Extraction Process[J]. Journal of Anhui Agricultural Sciences, 2010, 38(29): 16236-16238. DOI: 10.3969/j.issn.0517-6611.2010.29.065 |
| |
| Authors: | CHEN Xi |
| |
| Affiliation: | CHEN Xi(Key Laboratory of shaanci province,Shaanxi University of Technology Bio-resources,Hanzhong,Shaanxi 723000) |
| |
| Abstract: | [Objective]The research aimed to determine and compare the content of sinomenine in root and stem of caulis sinomenii and optimize the extraction process of it.[Method]HPLC was used to determine the content of sinomenine and orthogonal test was used to optimize the extraction process.[Result]The average content of sinomenine in root of caulis sinomenii was 1.73% which was 0.62% higher than that in stem.The optimum extraction process were as follows:ethanol concentration of 70%,ethanol volume of 15 ml,microw... |
| |
| Keywords: | Root of caulis sinomenii Sinomenine Determination of content Extraction process HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
