Nitric oxide-overproducing transformants of Pseudomonas fluorescens with enhanced biocontrol of tomato bacterial wilt |
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| Authors: | Yanqing Wang Qian Yang Yukio Tosa Hitoshi Nakayashiki Shigeyuki Mayama |
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| Affiliation: | (1) Laboratory of Plant Pathology, Faculty of Agriculture, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan;(2) Present address: South China Institute of Botany, Chinese Academy of Science, China |
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| Abstract: | The relation between nitric oxide (NO) production and the protective ability of Pseudomonas fluorescens T5 against bacterial wilt disease in tomato was examined. The endogenous nitric oxide reductase gene of T5 was disrupted by homologous recombination using a suicide plasmid. Three disruptants were obtained, and all had higher levels of NO production. Infection with Ralstonia solanacearum was reduced in tomato plants treated with the NO-overproducing transformants compared with the wild type. These results suggest that the modification of pseudomonads to increase their level of NO production is a new approach to enhancing their biocontrol efficacy. |
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| Keywords: | Pseudomonas fluorescens NO Nitric oxide reductase Bacterial wilt Ralstonia solanacearum |
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