The effect of pharmaceutical waste-fungal biomass,treated to degrade DNA,on the composition of eubacterial and ammonia oxidizing populations of soil |
| |
| Authors: | Maria Teresa Ceccherini Judith Ascher Giacomo Pietramellara Stefano Mocali Carlo Viti Paolo Nannipieri |
| |
| Institution: | (1) Dipartimento di Scienza del Suolo e Nutrizione della Pianta, Università degli Studi di Firenze, P.le delle Cascine 28, 50144 Firenze, Italy;(2) Istituto Sperimentale per la Nutrizione delle Piante, Sezione di Nutrizione Azotata e Microbiologia del Terreno, Via della Navicella 2/4, 00184 Roma, Italy;(3) Dipartimento di Biotecnologie Agrarie, Università degli Studi di Firenze, P.le delle Cascine 24, 50144 Firenze, Italy |
| |
| Abstract: | The aim of this work was to study variations in the composition of eubacteria and ammonia-oxidizing populations of soil, both
determined by denaturing gradient gel electrophoresis (DGGE), after the addition of a pharmaceutical fungal biomass, treated
to degrade its DNA. This waste can be used as an amendment. The fungal biomass waste was added at three rates: 0.05, 0.1,
and 1% per dry weight of soil. Control soil, without any amendment, was also investigated. Total DNA was extracted, purified,
and amplified by using either universal (eubacteria) or specific (amoA) primers. Amplicons were separated by DGGE. Sequencing was also carried out to better assess the diversity of ammonia oxidizing
bacteria. Changes in the composition of eubacterial community were detected after 3 days only in the soil treated with the
highest dose, while the ammonia oxidizing population responded more promptly (after 1 day) with evident modifications at level
of Nitrosolobus like sequences. |
| |
| Keywords: | DGGE Sequencing Soil DNA Molecular fingerprinting Ammonia-oxidizers Fungal pharmaceutical wastes |
| 本文献已被 SpringerLink 等数据库收录！ |
|
