朝鲜白头翁的组织培养与快繁技术

以朝鲜白头翁的叶片、叶柄和花梗为材料，研究了细胞分裂素zeatin、kinetin、6-BA 和生长素IAA 组合对诱导不定芽的影响；以不定芽为材料研究了植物生长调节剂对不定芽的增殖和生根的影响；研究了不同基质配比对组培苗移栽成活的影响。结果表明: 1）叶柄和花梗接种在MS + 0.5 mg · L^-1 zeatin +0.05 mg · L^-1 IAA 培养基培养6 周后不定芽再生率为100%。2）不定芽切成小块或单株带类似愈伤的组织块转到MS + 0.5 mg · L^-1 zeatin + 0.05 mg · L^-1 IAA 或MS + 1.0 mg · L^-1 6-BA + 0.05 mg · L^-1 IAA 的培养基时不定芽的增殖与生长良好。 3）在增殖培养基中长至3 cm 以上的小植株转入到1/2 MS + 1.5 mg · L^-1NAA的生根培养基中培养42 d 后，生根率高达93%，平均根长12.3 cm，每株平均根数11 条，且可缩短生根时间。4）组培苗移栽到东北山土和珍珠岩比例为2︰1 的混合基质时，成活率高达66.7%。

Studies on Tissue Culture and Rapid Propagation of Pulsatilla koreana Nakai

Leaf,petiole and pedicle explants of Pulsatilla koreana were cultured on MS medium supplemented with various concentrations of zeatin,kinetin or 6-BA combined with 0.05 mg·L~(-1) IAA.After 6 weeks of culture,effects of cytokinin on adventitious shoot formation from explants were investigated.The highest frequency of shoot formation was obtained when petiole and pedicle explants were cultured on MS+0.5 mg·L~(-1) zeatin+0.05 mg·L~(-1) IAA.Regenerated shoot were cut as several cluster or single plant with callus like tissue.and then transferred on MS+0.5 mg·L~(-1) zeatin+0.05 mg·L~(-1) IAA or MS+1.0 mg·L~(-1) 6-BA+0.05 mg-L~(-1) IAA for shoot propagation and growth.When shoot reached more than 3 cm in length,they were transferred on to root induction medium.93 percent of rooting ratio was observed in 1/2 MS+1.5 mg·L~(-1) NAA,after 42 days of culture.The rooted plantlets were acclimatized on mixture of North east mountain soil and perlite(2:1).