Role of p38 MAPK in cyclic mechanical stretch induced HMGB1 expression in alveolar macrophages |
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Authors: | DING Ning XIAO Hui GAO Ju XU Li-xin SHE Shou-zhang |
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Institution: | 1.Department of Anesthesiology and Anesthesiology Laboratory, 2Department of Out-patient, Guangzhou First Municipal People’s Hospital, Guangzhou 510180, China;3.Department of Anesthesiology, The Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China. E-mail: gzxulixin@163.com |
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Abstract: | AIM: To investigate the role of p38 mitogen-activated protein kinase (MAPK) in cyclic mechanical stretch induced the expression of high mobility group box 1 protein (HMGB1) in alveolar macrophages (AMs). METHODS: AMs were cultured and seeded at 1×108 cells/L in 6-well Bioflex cell culture plates. Subsequently, the cells were exposed to cyclic mechanical stretch at 20% (group B) elongation using Flexercell 4000T cell stretching unit. In group C, cells were pre-treated with SB203580 (40 μmol/L) for 2 h before mechanical stretch. Group A served as control. The expression of HMGB1 mRNA in alveolar macrophages was detected by RT-PCR. p38 MAPK activity and the expression of HMGB1 protein were measured by Western blotting analysis. RESULTS: The expression of HMGB1 mRNA and protein, and the activity of p38 MAPK in AMs were significantly increased in group B than those in group A (P<0.05). SB203580, an inhibitor of p38 MAPK, significantly inhibited the inducing effect of mechanical stretch (P<0.05). CONCLUSION: Mechanical stretch regulates the expression of HMGB1 mRNA and protein in alveolar macrophages by activating p38 MAPK signal pathway. |
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