Septic shock down-regulates L -arginine /NOS /NO pathway of platelet and aortic intima in rats |
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Authors: | CUI Yu-ying TANG Chao-shu GENG Bin FENG Hao-lou |
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Affiliation: | 1.Department of Physiology, Medical College of Hebei University, Baoding 071000, China;2.Institute of Cardiovascular Research, First Hospital of Peking University, Beijing 100034, China. E-mail:yuyingcui@sina.com |
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Abstract: | AIM: To investigate alteration and cross link of the aortic and platelet endogenous L -arginine/NOS/NO pathway induced by septic shock.METHODS: The septic shock model was made in rats by caecal ligation and puncture. NO-2/NO-3 production released from aortic and platelet was measured with Greiss assay. NOS activity and L-arginine transport activity were detected by isotope tracer method. RESULTS: Both in early and late stage of septic shock, NO-2/NO-3 production, NOS activity, and the L-arginine transport from the aorta intima and platelets were obviously decreased, while those of the aorta media and adventitia were obviously increased (P<0.01), but high-affinity L-arginine transport activity from the aorta intima and platelets was increased in early stage of septic shock (P>0.05 and P<0.05), as compared with the sham group, respectively. The inhibitory effects of NO-2/NO-3, NOS activity and the L-arginine transport showed a positive correlation between platelet and aortic intima (P<0.01). CONCLUSION: Septic shock down-regulates endogenous L-arginine/NOS/NO pathway in aortic intima and platelet, up-regulates L-arginine/NOS/NO pathway of aortic media and adventitia. Detection of the alteration of endogenous L-arginine/NOS/NO pathway in platelet might act as an indirect method to assess the endothelial dysfunction involving the pathogensis of septic shock. |
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Keywords: | Shock septic Tunica intima Blood platelets Nitric oxide Nitric-oxide synthase Arginine |
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