The feature of TCR-zeta chain expression in patients with CML by real-time quantitative PCR |
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Authors: | CHEN Si LI Yang-qiu CHEN Shao-hua YANG Li-jian WU Xiu-li CEN Dong-zhi |
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Affiliation: | Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China. E-mail:jnyangqiuli@163.com |
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Abstract: | AIM: To establish a real-time PCR technique for detection and quantification of TCR ζ chain expression and to investigate TCR ζ chain expression level in patients with chronic myeloid leukemia (CML). METHODS: Real-time PCR with SYBR GreenⅠ technology was used for detecting TCR ζ chain expression level in peripheral blood mononuclear cells from 30 patients with CML and 30 normal individuals. β2 -microglobulin gene (β2M) was used as an endogenous reference. Relative changes in TCR ζ chain expression level were used by the 2- △△Ct method between patients with CML and normal individuals. RESULTS: The SYBR GreenⅠ real-time technique for quantitative detection of TCR ζ chain expression levels was established successfully. The expression level of TCR ζ chain in 18 patients with CML was reduced. However, the TCR ζ chain expressed increased in 12 patients with CML. CONCLUSION: The TCR ζ chain expression level is divided into down expression (60%) and over expression (40%) groups, and the down expression of TCR ζ chain might related to cellular immunodeficiency in most of CML patients. |
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Keywords: | T-cell receptor ζ chain Real-time quantitative PCR Leukemia myeloid chronic |
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