The feature of TCR-zeta chain expression in patients with CML by real-time quantitative PCR

AIM: To establish a real-time PCR technique for detection and quantification of TCR ζ chain expression and to investigate TCR ζ chain expression level in patients with chronic myeloid leukemia (CML). METHODS: Real-time PCR with SYBR GreenⅠ technology was used for detecting TCR ζ chain expression level in peripheral blood mononuclear cells from 30 patients with CML and 30 normal individuals. β2 -microglobulin gene (β2M) was used as an endogenous reference. Relative changes in TCR ζ chain expression level were used by the 2- △△Ct method between patients with CML and normal individuals. RESULTS: The SYBR GreenⅠ real-time technique for quantitative detection of TCR ζ chain expression levels was established successfully. The expression level of TCR ζ chain in 18 patients with CML was reduced. However, the TCR ζ chain expressed increased in 12 patients with CML. CONCLUSION: The TCR ζ chain expression level is divided into down expression (60%) and over expression (40%) groups, and the down expression of TCR ζ chain might related to cellular immunodeficiency in most of CML patients.