MMP-7,MMP-10 and TIMP-4 expression in ventricular remodeling of human heart failure |
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Authors: | WEI Ying-jie HU Sheng-shou LI Jun ZHANG Xiao-ling CUI Chuan-jue HUANG Yin-xia SHEN Ya HUANG Jie ZHANG Hao |
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Institution: | Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, Cardiovascular Institute and Fu-Wai Hospital, PUMC and CAMS, Beijing 100037, China.E-mail:shengshouhu@yahoo.com |
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Abstract: | AIM:To sieve matrix metalloproteinases (MMPs) and the tissue inhibitors of matrix metalloproteinases (TIMPs) closely associated with ventricular remodeling of human heart failure using antibody chip technology.METHODS:We performed cytokine-specific antibody array analysis using individual left ventricular myocardial samples from 6 patients with heart failure due to arrythmogenic right ventricular cardiomyopathy (ARVC) undergoing transplantation and matched samples from 6 non-failing subjects to screen differentially expressed MMPs and TIMPs associated with the ventricular remodeling of heart failure.The results were further validated by ELISA and immunohistochemical analysis.RESULTS:We identified high expression of MMP-7 and MMP-10 and low expression of TIMP-4 in ARVC failing hearts compared to non-failing hearts by hybridization with the cytokine-specific antibody arrays containing 17 MMPs and 4 TIMPs on the chips.ELISA and immunohistochemical analyses further confirmed that differentially expressed levels of MMP-7, MMP-10, and TIMP-4 were observed not only in ARVC failing heart, but also in failing hearts due to ischemic (ICM) and dilated cardiomyopathy (DCM).CONCLUSION:Highly expressed MMP-7 and MMP-10 and lowly expressed TIMP-4 may be involved in the ventricular remodeling of heart failure derived from cardiomyopathy of different etiology. |
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