Effect of silencing hHBrk1 on proliferation and migration of lung carcinoma cells |
| |
Authors: | YU Hong-zhen ZHU Min ZHOU Ping-kun SUI Jian-li WANG Yu XU Qin-zhi WANG Si-ying |
| |
Affiliation: | 1.Department of Pathophysiology, College of Basic Medicine, Anhui Medical University, Hefei 230032, China;2.Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China. E-mail:sywan@ahmu.edu.cn |
| |
Abstract: | AIM: To observe the effect of hHBrk1 gene on proliferation and migration of lung carcinoma cells. METHODS: Recombinant plasmids harboring 19-nt-long small interfering RNA (siRNA) were constructed and tested to selectively downregulate hHBrk1 gene in human lung cancer 95D cell line in vitro by stable transfection with Lipofectamine 2000. The mRNA level of the cells transfected with siRNA plasmids were monitored by Northern blotting and RT-PCR. Growth curve and flow cytometry were applied to determine the cell proliferation and cell cycle. Ability of cell migration was measured by Trans-well system. RESULTS: hHBrk1 gene was silenced by targeting siRNA, and stable silencing cell model was constructed. No difference in proliferation and clone formation between hHBrk1 silencing cells and control cells was observed. The ability of migration was decreased in hHBrk1 silencing cells as compared with control cells. CONCLUSION: hHBrk1 may play an important role in migration of the lung cancer cells. |
| |
Keywords: | Genes hHBrk1 RNA interference Lung heoplasms Cell movement Cell proliferation Gene silencing |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
|
点击此处可从《园艺学报》下载全文 |
|