Insulin protects endothelial progenitor cells against functional damage caused by high glucose |
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Authors: | ZHAO Li WANG Hai-chang YIN Tao CHENG Kang GAO Feng |
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Institution: | 1.Department of Cardiology, Xijing Hospital,2Department of Physiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China. E-mail: wanghc@fmmu.edu.cn |
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Abstract: | AIM: To investigate the impact of various levels of glucose on endothelial progenitor cells (EPCs) proliferation, senescence, and nitric oxide (NO) secretion,and the effect of insulin under high glucose conditions.METHODS: Mononuclear cells were collected from rat bone marrow by density gradient centrifugation, cultured with medium 199, and identified to be EPCs at 7th day by flk-1 and AC133 double staining. EPCss were harvested and incubated with glucose (5, 10, 20, 40 mmol/L) or insulin (0.1, 1, 10, 100 nmol/L) under high glucose conditions for 24 h or 7 days. Proliferative capacity, senescence level and NO secretion (after 24 h of incubation) were subsequently determined.RESULTS: High glucose (40 mmol/L) markedly inhibited EPCs proliferation, accelerated EPCs senescence, and decreased NO production (all P<0.05). Compared with high glucose (40 mmol/L) group, insulin intervention promoted EPCs proliferation, inhibited EPCs senescence (prominent at 1 nmol/L, P<0.05), and enhanced NO secretion (prominent at 10 nmol/L, P<0.05).CONCLUSION: High glucose harms EPCs proliferation and function, while insulin alleviates this jeopardy, indicating the protective role of insulin for the cardiovascular system. |
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Keywords: | Glucose Insulin Endothelial progenitor cells Arteriosclerosis |
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