Protective effect of pentoxifylline against spinal cord ischemia/reperfusion injury in rabbits |
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Authors: | ZHU Dan-jie BI Qing XIA Bing HONG Jian-fei QIU Bin-song ZHANG Shui-jun |
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Affiliation: | Department of Orthopedics, Zhejiang Province People's Hospital, Hangzhou 310014, China. E-mail: zhudj@126.com |
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Abstract: | AIM: To investigate the protection of pentoxifylline against spinal cord ischemia/reperfusion injury.METHODS: Rabbits sustained spinal cord ischemia with 45 min cross-clamping of the infrarenal aorta. Groups were as follows: sham operation (n=8); ischemic control (n=20), receiving only vehicle; PTX A (n=20), receiving PTX before clamping and PTX B (n=20), receiving PTX at the onset of reperfusion. Rabbits were evaluated for hind-limb motor function with the Tarlov scoring system at 48 h. Serum was assayed with ELISA for TNF-α and spinal cords were harvest for MPO activity, histopathologic analysis and TUNEL staining. Immunohistochemistry was used for PECAM-1 and caspase-3 detection, and the numbers of necrosic and apoptotic neuron were counted at 12 h, 24 h, 48 h and 72 h of reperfusion. The necrotic and apoptotic neurons were also observed with transmission electron microscope.RESULTS: Improved Tarlov scores were observed in PTX-treated rabbits as compared with ischemic control rabbits at 48 h. The significant reductions of TNF-α in serum, activity of MPO, immunoreactivity of the PECAM-1 and caspase-3 were found in PTX-treated rabbits. The numbers of necrosic and apoptotic neuron were higher in PTX-treated rabbits than that in the ischemic control rabbits (P<0.05). No necrosic and apoptotic neuron were found in the sham operation group. CONCLUSION: PTX induces protection against ischemia/reperfusion injury in the spinal cord, thereby preventing both necrosis and apoptosis. |
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Keywords: | Reperfusion injury Pentoxifylline Spinal cord Rabbits Neurons |
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