Protein tyrosine phosphatase SHP-2 inhibits apoptosis induced by serum withdrawal in 293T cells

AIM: To investigate the potential role of Scy homology 2 domain-containing protein tyrosine phosphatase 2(SHP-2) in apoptosis of 293T cells induced by serum deprivation.METHODS: Transfection of plasmids into 293T cells was performed by liposome protocol. The viability of 293T cells was detected by MTT assay. On day 3 after removing serum the morphological changes of 293T cells were observed by using a transmission electron microscope, apoptosis rate was detected by flow cytometry and the expression of caspase-3 was measured by immunohistochemisty.RESULTS: The apoptosis rate in wild type SHP-2 (WT) group was obviously lower than that in the SHP-2C459S catalytically inactivated group. At the same time, expression of caspase-3 showed the similar results. CONCLUSION: SHP-2 may actively participate in the signal transduction pathway of apoptosis and play a positive role in cell survival. The underlying mechanism of apoptotic inhibition may be caspase-3 dependent.