Determination of immunochemically nonreactive urinary albumin by HPLC method and its significance of diagnose on children diabetic nephropathy |
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| Authors: | LI Mei-ai WAN Gen-ping LUO Ming-yong LIANG Xiao-yun ZHAO Xiao-yuan WU Dong-yan ZHANG Xiao-ling |
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| Institution: | 1.Department of Laboratory Medicine, 2Central Laboratory, The Children Hospital of Guangzhou, Guangzhou 510120, China. E-mail: limeiai740000@163.com |
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| Abstract: | AIM: To detect the immunochemically nonreactive urinary albumin and its significance of diagnose on diabetic nephropathy by high performance liquid chromatography (HPLC).METHODS: Urine samples of 58 diabetic children were collected. Urinary albumin was measured by HPLC and by immunoassay respectively.RESULTS: The retention times of albumin of standard and urine samples were 14.3 min. The content of albumin by HPLC and by immunoassay were 9.6-195.1 mg/L and 0-158.6 mg/L, respectively.The level of urinary albumin was higher by HPLC detection than that by immunoassay (P<0.05) in the same sample. The microalbuminuria rates were 70.7% detected by HPLC and 37.9% by immunoassay.CONCLUSION: Total urinary albumin can be measured by HPLC. Microalbuminuria in diabetic children can be observed earlier by HPLC. |
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