Effect of nicotine against apoptosis of rat cortical neurons induced by colchicines |
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Authors: | HUANG Xiao-hui ZHU Xiao-nan WANG Qin CHEN Ru-zhu WANG Xue-lan |
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Affiliation: | 1.Laboratory of Surgery, The First Affiliated Hospital, 2Department of Pharmacology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510080, China. E-mail: crz@mail.sysu.edu.cn |
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Abstract: | AIM:To explore the mechanism of nicotine against the apoptosis induced by colchicines in rat cortical neurons. METHODS:Cortical neurons were cultured from newborn Sprague-Dawley (SD) rats (less than 12 h). The rate of apoptosis was measured by Hoechst33258 fluorescence staining in the neurons, and the activity of Akt473 was analyzed by assay kit Akt473. RESULTS:The apoptosis of cortical neurons can be induced by 0.1 μmol/L colchicine. The phosphorlation of Akt 473 decreased significantly (1/3 times of the control group, P<0.01). However, when cortical neurons pretreated with 10 μmol/L nicotine for 2 h were cultured with 0.1 μmol/L colchicine for 24 h, the rate of apoptosis decreased from 62% to 38%. The phosphorlation of Akt473 increased significantly in a bell-shape time-dependent manner, which was respectively 1.3, 3.7, 2.4, 2.1 and 1.9 times compared with the control group (P<0.01). CONCLUSION:By activating the signal pathway of Akt473, nicotine may attenuate the apoptosis of cortical neurons induced by colchicines. |
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Keywords: | Nicotine Protein-serine-threonine kinases Cerebral cortex Neurons Colchicine |
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