The effect of TNF-related apoptosis-inducing ligand on the activity of nuclear kappa B in prostate cancer cell line PC-3M |
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Authors: | ZHONG Wei HE Wei MA Wang ZHANG Ke-liang WEI Shao-zhong CHEN Zhao-hui CHEN Xiao-chun ZENG Fu-qing XIAO Chuan-guo△ |
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Affiliation: | 1.Institute of Urology,Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;2.Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;3.Department of Surgery, Hubei Cancer Hospital, Wuhan 430079,China. E-mail: bellway@163.com |
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Abstract: | AIM: To investigate the activation and inactivation of nuclear factor kappa B (NF-κB) when tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is applied to induce the apoptosis of androgen-independent prostate cancer cell line PC-3M.METHODS: After the treatment of TRAIL or LPS at different doses, we tested the nuclear translocation of NF-κB by cell immunohistochemical staining and electrophoretic mobility shift assay(EMSA), and evaluated the level of IκB by RT-PCR under pyrrolidine dithiocarbamate (PDTC) treatment. RESULTS: EMSA and cell immunohistochemical analysis showed that the translocation of NF-κB was significantly activated when PC-3M cells were treated with TRAIL or LPS (P<0.05). The pretreatment of PDTC upregulated the expression of IκB and blocked the nuclear translocation of NF-κB.CONCLUSION: TRAIL remarkably stimulates the activation of nuclear NF-κB in androgen-independent prostate cancer cells. On the other hand, the translocation of NF-κB can be significantly and efficiently inhibited in PC-3M cells by pretreatment with PDTC. The increased expression of IκB might be a clue for this inhibition, which means the possible way to enhance the effect of TRAIL in the apoptosis of prostate cancer cells. |
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Keywords: | Prostatic neoplasms Tumor necrosis factor NF-kappa B Tumor necrosis factor-related apoptosis-inducing ligand |
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