Maturity and storage influence on the apple (Malus domestica) allergen Mal d 3, a nonspecific lipid transfer protein |
| |
Authors: | Sancho Ana I Foxall Robert Rigby Neil M Browne Thomas Zuidmeer Laurian van Ree Ronald Waldron Keith W Mills E N Clare |
| |
Institution: | Institute of Food Research, Norwich Research Park, Colney, Norwich NR47UA, United Kingdom. ana.sancho@bbsrc.ac.uk |
| |
Abstract: | Consumption of apples can provoke severe allergic reactions, in susceptible individuals, due to the presence of the allergen Mal d 3, a nonspecific lipid transfer protein, found largely in the fruit skin. Levels of Mal d 3 were determined in peel as a function of apple cultivar, position of the fruit growing on the tree, apple maturity, and postharvest storage by ELISA. As the apples mature, Mal d 3 levels increased, although the rate was dependent on cultivar and tree position. During storage, levels of Mal d 3 decreased in all cultivars (cvs. Cox, Jonagored, and Gala), the rate of overall decrease being greatest under controlled atmosphere conditions. There was no correlation between Mal d 3 levels and total apple peel protein, indicating specific alterations in Mal d 3 expression. Thus pre- and postharvest treatments (i.e., storage) can modify the allergen load in apple peel, the highest levels being found in overly mature and freshly harvested fruits. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|