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| 金花茶皂甙分离纯化的初步研究 | |
| 引用本文： | 林华娟,秦小明,宁恩创,曾秋文,杨继柱.金花茶皂甙分离纯化的初步研究[J].广西热带农业,2009(2). |
| 作者姓名： | 林华娟 秦小明 宁恩创 曾秋文 杨继柱 |
| 作者单位： | 1. 广东海洋大学食品科技学院,湛江,524088 2. 广西大学轻工与食品工程学院,南宁 3. 广西富新科技股份有限公司,防城港 |
| 基金项目： | 广西科技厅科技合作与交流项目，广西大学科研基金，防城港市科技攻关与新产品试制项目 |
| 摘 要： | 以金花茶叶浓缩液为试验材料,探讨了金花茶皂甙的分离纯化方法.实验结果表明,乙酸乙酯萃取的方法不能有效将皂甙与茶多酚分离;采用Diaion HP-10和HPD-450两种大孔吸附树脂均可以将金花荼皂甙分离成S 1、S 2、S 3和S 4等4个组分,其中S 1组分的皂甙含量分别为96.4%和93.6%;Diaion HP-10大孔树脂对茶多酚有较强的吸附力. |
| 关 键 词： | 金花茶 皂甙 茶多酚 分离纯化 |
Preliminary Study on Isolation and Purification of Saponin from the Leaves of Camellia chrysantha(Hu)Tuyama |
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| Lin Hua-juan,Qin Xiao-ming,Ning En-chuang,Zeng Qiu-wen,Yang ji-zhu.Preliminary Study on Isolation and Purification of Saponin from the Leaves of Camellia chrysantha(Hu)Tuyama[J].Guangxi Tropical Agriculture,2009(2). | |
| Authors: | Lin Hua-juan Qin Xiao-ming Ning En-chuang Zeng Qiu-wen Yang ji-zhu |
| Institution: | 1 College of Food Science and Technology;Guangdong Ocean University;Zhanjiang 524088;China;2College of Light Industry and Food Engineering;GuangXi University;Nanning;3Guangxi Fuxin Technological Stock Co.;Fangchenggang |
| Abstract: | Using concentrated solution of Camellia chrysantha(Hu)Tuyama as material,isolation and purification methods of saponin was discussed.The results showed that the solvent extraction with ethyl acetate could not separate saponin from tea polyphenol.Saponin were separated into S1,S2,S3 and S4 fractions by using either of Diaion HP-10 and HPD-450 resin,and the saponin content of S1 fractions were 96.4% and 93.6% respectively.Diaion HP-10 resin has stronger adsorption capacity to tea polyphenol than that of HPD-4... |
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