| 白花败酱组织培养及快速繁殖研究 |
| |
| 引用本文： | 庾韦花,张向军,蒙平,蒋慧萍,陈少珍. 白花败酱组织培养及快速繁殖研究[J]. 北方园艺, 2009, 0(8) |
| |
| 作者姓名： | 庾韦花 张向军 蒙平 蒋慧萍 陈少珍 |
| |
| 作者单位： | 广西农业科学院,广西,南宁,530007 |
| |
| 摘 要： | 选取长势较好、无病害野生白花败酱植株的茎尖及茎段作为外植体进行组织培养,以MS为基本培养基,设计不同激素浓度配比试验,筛选最佳培养基配方。结果表明:采用0.1%HgCl2对外植体进行消毒,茎尖最佳消毒时间为10 min,茎段为15 min;最佳诱导培养基配方是MS+6-BA 3.0 mg/L+NAA 0.1 mg/L,诱导率100%,增殖培养基MS+6-BA 2.0 mg/L+NAA0.2 mg/L,增殖倍数7.8;生根壮苗培养基是MS+NAA 1 mg/L+马铃薯5%。
|
| 关 键 词： | 白花败酱 茎尖 茎段 诱导 生根 |
Tissue Culture and Rapid Propagation of Patrinia villasa Juss |
| |
| YU Wei-hua,ZHANG Xiang-jun,MENG Ping,JIANG Hui-ping,CHEN Shao-zhen. Tissue Culture and Rapid Propagation of Patrinia villasa Juss[J]. Northern Horticulture, 2009, 0(8) |
| |
| Authors: | YU Wei-hua ZHANG Xiang-jun MENG Ping JIANG Hui-ping CHEN Shao-zhen |
| |
| Affiliation: | Guangxi Academy of Agricultural Sciences;Nanning;Guangxi 530007;China |
| |
| Abstract: | Took the shoot tips of well-grown,virus-free wild Patrinia villasa Juss as explants,the micropropagation system of Patrinia villasa Juss was established.The research showed that,the prefer able sterilized protocol was to take 15 min of shoot length as explants,and surface sterilized with 0.1% HgCl2 solution for 10 min.The most effective induction and subculture media were MS basal medium supplemented with 3.0 mg/L BA and 0.1 mg/L NAA which got 100% induction frequency,with 2.0 mg/L BA and 0.2 mg/L NAA which... |
| |
| Keywords: | Patrinia villasa Juss Stem tip and shoot Induction Rooting |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
