三种复方中草药对鲫血清、肝胰脏及脾脏中溶菌酶活性的影响

在水温18℃下研究了三种复方中药（AB、CD、AH）作为饲料免疫添加剂对体质量（44.6±0.2）g 1＋龄健康鲫（Carassius auratus）血清、肝胰脏和脾脏中溶菌酶活性的影响。试验鱼分为3组,每天按体质量的2.0%分别投喂添加了3种中药复方的饲料,对照组投喂基础饲料,每组均设相同数量的鱼做平行,连续饲喂28 d,停食1d后采集血液及组织样品,测定溶菌酶活性。结果显示：3种复方中药都能提高鲫体内溶菌酶活性。其中AB组提高血清中溶菌酶活性效果显著,AB、CD组对肝胰脏中溶菌酶活性的提升效果显著,3种复方中药对鲫脾脏中溶菌酶活性的提升均效果显著,说明3种复方中药均可增强鲫免疫力,有进一步研究和应用的价值,但对不同组织溶菌酶活性的影响不同,提示中药作用效果和机理的差异。

The Effects of Three Traditional Chinese Medicine Compounds on Lysozyme Activities in Serum,Hepatopancreas and Spleen of Crucian Carp

The effects of three traditional Chinese medicine compounds（AB,CD,and AH） on lysozyme activities in serum,hepatopancreas and Spleen were studied in 1＋ age health crucian carp（Carassius auratus） weighing（44.6±0.2） g were studied at water temperature of 18 ℃.The fish were fed the diets containing AB,CD,AH or basic diet without any Chinese medicine compounds at a feeding rate of 2.0% for 28 days.Blood and tissue samples were collected 1 day after fasting to be assayed for the lysozyme activity.The results showed that the three Chinese medicine compounds improved the lysozyme activity in the crucian carp.There was significant increase in serum lysozyme activity in the fish fed the diet containing AB than that in the control group.The fish fed the diets containing AB,and CD had significant enhancement of hepatopancreas lysozyme activity.However,significant higher lysozyme activity was found in the spleen of the fish fed the diet containing the three traditional Chinese medicine compounds.It is concluded that the traditional Chinese medicine compounds can enhance crucian carp immunity,and have a value for further research and application.Still,the medicine mechanism and functions are needed for further research due to organ and tissue specialization for the three compounds.