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山西地区褐土胶质芽孢杆菌生物学特性研究
引用本文:张晓波,赵艳,樊俊华.山西地区褐土胶质芽孢杆菌生物学特性研究[J].草原与草坪,2010,30(4):89-92,96.
作者姓名:张晓波  赵艳  樊俊华
作者单位:1. 海南大学,农学院,海南,儋州,571737
2. 山西农业大学,资源环境学院,山西,太谷,030801
基金项目:海南省自然科学基金(309009)"结缕草根际联合固氮菌的分离、鉴定及筛选",海南省教育厅高等学校科研项目(Hjkj2010-16)"海南省不同暖季型草坪草根际胶质芽孢杆菌遗传多样性及解钾活性的研究"资助 
摘    要:从山西地区褐土中筛选出6株胶质芽孢杆菌,以辽宁菌种保藏中心提供的胶质芽孢杆菌(编号K0)为参照菌株,对其形态学、生理生化特性、耐盐性、耐酸碱性、温度敏感性等特性进行了测定。结果表明,这些菌株均为革兰氏阴性杆菌,产生圆形芽孢和丰厚的荚膜,菌落透明隆起,粘性强;过氧化氢酶试验呈阳性,能水解淀粉,VP阴性,不产生吲跺。K5、K0在3%NaCl浓度的培养基上能生长;在25~30℃供试胶质芽孢杆菌均能良好生长。研究可为高效胶质芽孢杆菌菌株材料的获得以及研制生物钾肥提供实践及理论基础。

关 键 词:胶质芽孢杆菌  褐土  生物学特性

Study on biological characteristics of Bacillus mucilaginosus isolated from cinnamon soil in Shanxi
ZHANG Xiao-bo,ZHAO Yan,FAN Jun-hua.Study on biological characteristics of Bacillus mucilaginosus isolated from cinnamon soil in Shanxi[J].Grassland and Turf,2010,30(4):89-92,96.
Authors:ZHANG Xiao-bo  ZHAO Yan  FAN Jun-hua
Institution:1.College of Agronomy,Hainan University,Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources(Hainan University),Ministry of Education,Danzou 571737,China;2.College of Resources and Environment,Shanxi Agricultural University,Taigu 030801,China)
Abstract:Six strains of Bacillus mucilaginosus were screened from cinnamon soil in Shanxi,and the physiological and biochemical characters,salt tolerance,acid and alkali resistance,and temperature sensitivity were determined by using the modal strain(strain code:K0) from Liaoning Center for Microorganism Reserve.The results showed that all strains were gram negative bacteria with circular gemma and thick capsule;the catalase test was masculine.They could hydrolyze starch and the V.P test was feminine.Strains K5 and K0 could survive in the medium with 3% NaCl and grow well under the temperature ranging from 25 ℃ to 30 ℃.The result could provide basis to obtain efficient strains for bio-fertilizer development.
Keywords:Bacillus mucilaginosus  cinnamon soil  physiological and biochemical character
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