| 四川竹根姜胚性愈伤组织诱导和植株再生 |
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| 引用本文： | 郭英华,张振贤,关秋竹.四川竹根姜胚性愈伤组织诱导和植株再生[J].河北农业大学学报,2005,28(6):36-39. |
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| 作者姓名： | 郭英华 张振贤 关秋竹 |
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| 作者单位： | 中国农业大学,农学与生物技术学院,北京,100094;中国农业大学,农学与生物技术学院,北京,100094;中国农业大学,农学与生物技术学院,北京,100094 |
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| 基金项目： | 国家科技攻关项目资助（2001BA511B-08-12）;农业部农业结构调整项目（04-06-02B） |
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| 摘 要： | 选用四川竹根姜的茎尖组织,诱导产生了胚性愈伤组织,建立了高频再生体系。筛选出了适于胚性愈伤诱导及扩增最佳培养基MSN+1.0 mg/L 2,4-D+0.2 mg/L KT+3.0%蔗糖+0.7%琼脂;胚性愈伤组织分化培养基MS+0.2 mg/L 2,4-D+5.0 mg/L BA+3.0%蔗糖+0.7%琼脂;根状茎形成培养基MS+3.0 mg/LBA+0.1 mg/L NAA+6.0%蔗糖+0.7%琼脂。在3.0%~6.0%的范围内蔗糖浓度越高越促进根状茎形成。
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| 关 键 词： | 生姜 体细胞胚 愈伤组织 植株再生 |
| 文章编号： | 1000-1573（2005）06-0036-04 |
| 收稿时间： | 2005-03-18 |
| 修稿时间： | 2005-03-18 |
Somatic and embryogenic callus induction and plant regeneration from ginger(Zingiber officinale Rosc.) shoot-tips of Zhugen gingers from Sichuan province |
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| GUO Ying-hua,ZHANG Zhen-xian,GUAN Qiu-zhu.Somatic and embryogenic callus induction and plant regeneration from ginger(Zingiber officinale Rosc.) shoot-tips of Zhugen gingers from Sichuan province[J].Journal of Agricultural University of Hebei,2005,28(6):36-39. |
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| Authors: | GUO Ying-hua ZHANG Zhen-xian GUAN Qiu-zhu |
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| Institution: | College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China |
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| Abstract: | The system of induction and plant regeneration from somatic and embryogenic callus of Zhugen ginger was established.Somatic and embryogenic callus was induced from ginger shoot tips on MS medium supplemented with 1.0 mg/L 2,4-D and 0.2 mg/L KT,which contained only half concentration of NH_4NO_3.The callus(about 1.0 cm in diameter) was transferred on MS medium containing 0.2 mg/L 2,4-D,5.0 mg/L BA,3.0% sucrose and 0.7% agar,and shoots and roots were formed.And shoots developed into complete plantlets with rhizomes on MS medium supplemented with 3.0 mg/L BA and 0.1 mg/L NAA and 6.0% sucrose.The effects of phytohormone and nitrogen on induction and redifferenciation of embryogenic callus and the effect of sucrose on rhizome formation were all researched. |
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| Keywords: | ginger embryogenesis callus plant regeneration |
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