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AgNO_3对梨叶片不定芽诱导过程中内源多胺含量的影响
引用本文:刘娟,汤浩茹,刘玲梅. AgNO_3对梨叶片不定芽诱导过程中内源多胺含量的影响[J]. 果树学报, 2008, 25(6): 920-923,F0004
作者姓名:刘娟  汤浩茹  刘玲梅
作者单位:[1]四川农业大学林学园艺学院,四川雅安625014 [2]四川省农业厅经济作物处,成都610041
基金项目:国家自然科学基金"西南地区树莓植物遗传多样性研究与特异资源筛选",教育部"新世纪优秀人才支持计划",高等学校全国百篇优秀博士学位论文作者专项基金
摘    要:为了探讨AgNO3在离体培养物形态发生中的作用,以金水梨组培苗为外植体,研究了不同浓度AgNO3对梨叶片再生不定芽过程中内源多胺含量的影响。结果表明,多胺含量会随着外植体细胞分裂分化迅速增加,其中腐胺含量在培养5d时形成高峰,精胺在20d时出现峰值,而亚精胺在培养过程中2次出现峰值。经AgNO3处理后叶片内源多胺含量与对照相比明显增多,其中以0.1mg/LAgNO3处理效果最明显。

关 键 词:  叶片培养  AgNO3  内源多胺

Effects of AgNO_3 on changes of endogenous polyamine contents in the process of adventitious bud formation in in vitro leaves of pear
LIU Juan,,TANG Hao-ru,LIU Ling-mei. Effects of AgNO_3 on changes of endogenous polyamine contents in the process of adventitious bud formation in in vitro leaves of pear[J]. Journal of Fruit Science, 2008, 25(6): 920-923,F0004
Authors:LIU Juan    TANG Hao-ru  LIU Ling-mei
Affiliation:LIU Juan1,2,TANG Hao-ru1,LIU Ling-mei1(1College of Forestry , Horticulture,Sichuan Agricultural University,Ya'an,Sichuan 625014 China,2Department of Economic Crops,Agricultural Bureau of Sichuan Province,Chengdu,Sichuan 610041 China)
Abstract:Effects of different AgNO3 concentrations on the content of endogenous polyamine in in vitro leaves of Pyrus brestchneideri Rehd.cv.Jinshui was studied in order to understand the effect of AgNO3 in the process of in vitro morphologe-nesis.The results indicated that the content of endogenous polyamine would be enriched with cell division and differentiation.The content of putrescine(Put) and spermine(Spm) formed pinnacles on 5th day and 20th day respectively,while the content of spermindine(Spd) was enhanced...
Keywords:AgNO3
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