| Genetic Analysis and Molecular Mapping of Two SMV-Resistance Traits in Soybean： Adult-Plant Resistance and Resistance to Seed Coat Mottling |
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| 作者单位： | Agriculture Academy,Northeast Agricultural University/Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province/ National Research Center of Soybean Engineering and Technology/Soi |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展规划(973计划)，the National 863 Program of China，the National 948 Project of China |
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| 摘 要： | Soybean mosaic virus （SMV） could lead to adult-plant system diseases, and cause mottling of soybean seeds. Genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from two crosses of Dongnong 3C624 （susceptible）x Dongnong 8143 （resistant） and Dongnong 3C628 （susceptible）× Tie 6915 （resistant）. Simple sequence repeat （SSR） markers with bulked segregation analysis （BSA） were used to conduct genetic mapping of the resistance to SMV1 in the segregating populations. The results indicated that resistance to SMV1 in adultplants and the resistance to seed coat mottling in Dongnong 8143 and Tie 6915 was separately controlled by one single dominant gene. The two dominant genes were identified to be linked on the MLG F by Mendel＇s genetics and SSR genetic mapping. The order and distance of markers DPRSMV1 and DSRSMV1 were Sat 229-6.9 cM-DSRSMV1-4.6 cM-Sat_317-3.6 cM-DPRSMV1-5.2 cM-Satt335. The order and distance of markers TPRSMV1 and TSRSMV1 was Satt160-16.1 cM-TPRSMV1-7.3 cM-Satt516-2.0 cM-TSRSMV1-4.5 cM-Sat_133. This research provides the useful information for breeders to select the two types of SMV resistance simultaneously in soybean breeding through molecular marker-assisted selection （MAS）.
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| 关 键 词： | 分子标记辅助选择 大豆花叶病毒 抗性性状 遗传分析 种粒斑驳 静脉 显性基因控制 映射 |
| 收稿时间： | 2009-03-30 |
Genetic Analysis and Molecular Mapping of Two SMV-Resistance Traits in Soybean: Adult-Plant Resistance and Resistance to Seed Coat Mottling |
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| WANG Kun,LI Wen-fu,ZHANG Lei,LIU Chun-yan,ZHU Xiao-shuang,CHEN Qing-shan,HU Guo-hua. Genetic Analysis and Molecular Mapping of Two SMV-Resistance Traits in Soybean: Adult-Plant Resistance and Resistance to Seed Coat Mottling[J]. 《Agricultural Sciences in China》, 2010, 9(1): 11-18. DOI: 10.1016/S1671-2927(09)60062-6 |
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| Authors: | WANG Kun LI Wen-fu ZHANG Lei LIU Chun-yan ZHU Xiao-shuang CHEN Qing-shan HU Guo-hua |
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| Affiliation: | Agriculture Academy,Northeast Agricultural University/Crop Research and Breeding Center of Land-Reclamation of Heilongjiang Province/ National Research Center of Soybean Engineering and Technology/Soi |
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| Abstract: | Soybean mosaic virus (SMV) could lead to adult-plant system diseases, and cause mottling of soybean seeds. Genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from two crosses of Dongnong 3C624 (susceptible) ×Dongnong 8143 (resistant) and Dongnong 3C628 (susceptible) × Tie 6915 (resistant). Simple sequence repeat (SSR) markers with bulked segregation analysis (BSA) were used to conduct genetic mapping of the resistance to SMV1 in the segregating populations. The results indicated that resistance to SMV1 in adult-plants and the resistance to seed coat mottling in Dongnong 8143 and Tie 6915 was separately controlled by one single dominant gene. The two dominant genes were identified to be linked on the MLG F by Mendel's genetics and SSR genetic mapping. The order and distance of markers DPRSMV1 and DSRSMV1 were Sat229-6.9 cM-DSRSMV1 -4.6 cM-Sat_317-3.6 cM-DPRSMV1-5.2 cM-Satt335. The order and distance of markers TPRSMV1 and TSRSMV1 was Sattl60-16.1 cM-TPRSMV1-7.3 cM-Satt516-2.0 cM-TSRSMV1-4.5 cM-Sat_133. This research provides the useful information for breeders to select the two types of SMV resistance simultaneously in soybean breeding through molecular marker-assisted selection (MAS). |
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| Keywords: | soybean SMV seed coat mottling genetic analysis SSR gene mapping |
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