| 总丹参酮超声提取及其主要成分的HPLC法测定 |
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| 引用本文： | 张源源,田友清.总丹参酮超声提取及其主要成分的HPLC法测定[J].安徽农业科学,2011,39(4):2083-2084. |
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| 作者姓名： | 张源源 田友清 |
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| 作者单位： | 江苏联合职业技术学院连云港中医药分院,江苏连云港,222006;江苏联合职业技术学院连云港中医药分院,江苏连云港,222006 |
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| 基金项目： | 连中医高职校创新基金课题 |
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| 摘 要： | 目的]提取总丹参酮并测定其中3种主要成分的含量。方法]运用超声技术提取总丹参酮,并对提取物中隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA含量进行HPLC测定。结果]总丹参酮提取物中隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA成分完全分离,含量分别为100.4、69.9和124.4 mg/g。结论]HPLC法简便快速,稳定可靠,对总丹参酮提取物的质量控制具有一定的参考价值。
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| 关 键 词： | 总丹参酮 超声提取 隐丹参酮 丹参酮Ⅰ 丹参酮ⅡA 高效液相色谱 |
Ultrasonic Extraction and HPLC Determination of Main Contents of Total Tanshinone |
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| ZHANG Yuan-yuan et al.Ultrasonic Extraction and HPLC Determination of Main Contents of Total Tanshinone[J].Journal of Anhui Agricultural Sciences,2011,39(4):2083-2084. |
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| Authors: | ZHANG Yuan-yuan |
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| Institution: | ZHANG Yuan-yuan et al(Lianyungang Chinese Medicine Branch of Jiangsu United Vocational College,Lianyungang,Jiangsu 222006) |
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| Abstract: | Objective]The aim was to extract total tanshinone and determine three main contents of total tanshinone.Method]Total tanshinone was extracted based on the methods of ultrasonic technique,moreover,three main tanshinone contents,namely,cryptotanshinone and tanshinone Ⅰas well as tanshinone ⅡA,were determined by using HPLC method.Result]The composition of cryptotanshinone,tanshinone Ⅰ,tanshinone ⅡA were separated completely from total tanshinone extraction and the contents were 90.3,61.7 and 109.8 mg/g,respectively.Conclusion]HPLC method was simple,fast,stable and reliable,which provided certain reference value for the quality control of total tanshinone extraction. |
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| Keywords: | Total tanshinone Ultrasonic extraction Cryptotanshinone Tanshinone Ⅰ Tanshinone ⅡA HPLC |
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