| 球等鞭金藻OA-3011积累脂肪酸的条件研究 |
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| 引用本文： | 刘云鹤,张钱宝,周鸣谦,许兵. 球等鞭金藻OA-3011积累脂肪酸的条件研究[J]. 安徽农业科学, 2011, 39(4): 1951-1953 |
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| 作者姓名： | 刘云鹤 张钱宝 周鸣谦 许兵 |
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| 作者单位： | 淮海工学院海洋学院,江苏连云港,222005;江苏省海洋生物技术重点实验室,江苏连云港,222005;淮海工学院海洋学院,江苏连云港,222005 |
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| 基金项目： | 江苏省产学研联合创新资金项目，江苏省海洋生物技术重点实验室开放课题，江苏淮海工学院校内自然科学基金项目 |
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| 摘 要： | 以球等鞭金藻OA-3011培养液的细胞密度和脂肪酸含量为评价指标,利用均匀设计确定了硝酸钠和磷酸二氢钾的用量;选择影响球等鞭金藻积累脂肪酸的4个因素进行3水平的正交试验,确定其积累脂肪酸的最优条件。结果表明,海水培养基中硝酸钠的适宜浓度为75 mg/L,磷酸二氢钾的适宜浓度为8 mg/L;球等鞭金藻OA-3011的最佳培养条件为：盐度30‰,pH值7.5,接种量90×104个/ml,培养温度25℃,此条件下培养液中脂肪酸含量可达48 mg/L。
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| 关 键 词： | 球等鞭金藻 脂肪酸 细胞密度 |
Study on Accumulation Condition of Fatty Acid in Isochrysis galbana OA-3011 |
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| LIU Yun-he et al. Study on Accumulation Condition of Fatty Acid in Isochrysis galbana OA-3011[J]. Journal of Anhui Agricultural Sciences, 2011, 39(4): 1951-1953 |
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| Authors: | LIU Yun-he et al |
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| Affiliation: | LIU Yun-he et al(Ocean College of Huaihai Institute of Technology,Lianyungang,Jiangsu 222005) |
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| Abstract: | With cell density and fatty acid content in culture medium of Isochrysis galbana OA-3011 as evaluation indexes,the dosages of sodium nitrate and potassium dihydrogen phosphate were ascertained using uniform design.Four influencing factors of fatty acid accumulation in I.galbana were selected for 4-factor and 3-level orthogonal test so as to ascertain the optimum condition for its fatty acid accumulation.Results showed that the suitable concn.of sodium nitrate and potassium dihydrogen phosphate in sea water ... |
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| Keywords: | Isochrysis galbana Fatty acid Cell density |
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