| 延边黄牛气肿疽间接ELISA诊断方法的建立 |
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| 引用本文： | 车达,金鑫,陈莹莹,费景春,姜丹丹,吴丹,王笑玉.延边黄牛气肿疽间接ELISA诊断方法的建立[J].安徽农业科学,2011,39(2):1042-1044. |
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| 作者姓名： | 车达 金鑫 陈莹莹 费景春 姜丹丹 吴丹 王笑玉 |
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| 作者单位： | 车达,金鑫,陈莹莹,姜丹丹(延边大学农学院,吉林延吉,133400);费景春(吉林松原职业技术学院,吉林松原,138000);吴丹,王笑玉(吉林省动物疫病预防控制中心,吉林长春,130062) |
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| 摘 要： | 目的]检测延边黄牛气肿疽病原体,为牛气肿疽的检测提供科学方法和依据。方法]采用气肿疽梭菌裂解菌体为抗原,建立检测该病原菌的酶联免疫吸附检测方法,确定其最适工作条件。结果]气肿疽梭菌菌体裂解抗原最适包被浓度为50μg/m l;待检血清的最适稀释度为1∶200,酶标二抗的最适工作浓度为1∶2 000;对随机采集于延边地区的30份牛血清样品进行间接ELISA检测,阳性率为20%。结论]建立的间接ELISA方法特异性和重复性好。
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| 关 键 词： | 延边黄牛 气肿疽梭菌 菌体抗原 间接ELISA |
Development of Indirect ELISA for Clostridium chauvoei Infection in Yanbian Cattle |
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| Institution: | CHE Da et al (Gricultural College of Yanbian University,Yanji,Jilin 133400) |
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| Abstract: | Objective] To develop an indirect ELISA for detecting Clostridium chauvoei pathogen in Yanbian cattle.Method] Clostridium chauvoei was used as antigen,ELISA detection method was established and the optimum working conditions were also determined.Result] The optimum concentration of Clostridium chauvoei thallus antigen for coating of plate was 50 μg/ml,the dilution of serum sample was 1∶200,the working concentration of HRP-labeled rabiit anti-bovine IgG was 1∶2 000.Moreover,all the 30 bovine serum samples collected from Yanbian canton were detected by the indirect ELISA and found the positive rate was 20%.Conclusion] The indirect ELISA method is specific and reproducible. |
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| Keywords: | Yanbian cattle Clostridium chauvoei Thallus antige Indirect ELISA |
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