两蜂种蜂王浆蛋白质组分析

采用蛋白质组双向电泳技术和已鉴定蜂王浆蛋白质组的比对，对王浆高产蜜蜂（浆蜂）和中华蜜蜂（中蜂）的蜂王浆蛋白质组进行了差异比较。结果表明，浆蜂蜂王浆的总蛋白质点数（93个）显著高于中蜂蜂王浆总蛋白质点数（70个），它们的等电点主要集中在5-8之间，分子量在50-80kDa之间，其中共有蛋白质点为30个；通过与已鉴定的浆蜂蜂王浆蛋白质组比较，这些共有蛋白质点推断为蜂王浆主蛋白1、2、3和葡萄糖氧化酶。同时两蜂种蜂王浆的蛋白质丰度也存在较大差异，共有点中有4个蛋白质的丰度中蜂显著高于浆蜂，7个浆蜂显著高于中蜂中。结果表明浆蜂和中蜂蜂王浆蛋白质种类及丰度均有较大差异。

Analysis of royal jelly proteome of two kinds honeybee

Proteome difference between high royal jelly producing bees (A. mellifera L.) and Chinese honeybees (A. cerana cerana F.) was investigated by two-dimensional gel electrophoresis, and some proteins were deduced by comparing with the already identified royal jelly proteome . Significant higher protein spots (93) in royal jelly of high royal jelly producing bee were found than those of in Chineses honeybee’s (70). Their molecular weight ranged from 50 to 80 kDa, and pI 5.0-8.0. Thirty protein spots were resolved to two images, while others were unique to each sample. Comparing with the already identified royal jelly proteome, it can be deduced that these well resovled proteins were likely MRJP1 (major royal jelly protein 1), MRJP2, MRJP3 and glucose oxidase. Protein abundance analysis of proteins present on two samples showed that 4 proteins of Chinese honeybee significantly higher than those of high royal jelly producing bees, but 7 spots showed a reverse relation. The results suggest that there are significant differences between royal jelly from high royal jelly producing bees and Chinese honeybees in term of proteins composition and abundance.